Asian Journal of Biology <p style="text-align: justify;">The aim of <strong>Asian Journal of Biology&nbsp;(ISSN: 2456-7124)</strong>&nbsp;is to publish high quality papers (<a href="/index.php/AJOB/general-guideline-for-authors">Click here for Types of paper</a>) with broad areas of Aerobiology, &nbsp;Agriculture, Anatomy, Astrobiology, Biochemistry, Bioengineering, Bioinformatics, Biomathematics or Mathematical Biology, Biomechanics, Biomedical research, Biophysics, Biotechnology, Building biology, Botany, Cell biology, Conservation Biology, Cryobiology, Developmental biology, Food biology, Ecology, Embryology, Entomology, Environmental Biology, Epidemiology, Ethology, Evolutionary Biology, Genetics, Herpetology, Histology, Ichthyology, Integrative biology, Limnology,&nbsp; Mammalogy, Marine Biology, Microbiology, Molecular Biology, Mycology, Neurobiology, Oceanography, Oncology, Ornithology, Population biology, Population ecology, Population genetics, Paleontology, Pathobiology or pathology, Parasitology, Pharmacology, Physiology, Psychobiology, Sociobiology, Structural biology, Virology and&nbsp; Zoology. This is a quality controlled, peer-reviewed, open access INTERNATIONAL journal.</p> en-US (Asian Journal of Biology) (Asian Journal of Biology) Tue, 28 Jul 2020 07:45:10 +0000 OJS 60 Biodiversity of Soil Microorganisms and their Effects on Disease Management at Black Pepper Farms in Gia Lai Province <p>Research on soil ecological systems, such as the soil characteristics and properties of the soil micro-flora system, is essential in the sustainable production of black pepper (<em>Piper nigrum </em>L.).The role of using beneficial microorganisms in the sustainable production of black pepper is in increase because many people are being aware of the hazards associated with consuming products with chemical substances. Twenty-seven soil samples were collected from Chu Se, Chu Prong and Duc Co district of Gia Lai province to determine the relationships between the ecological system, pathogenic fungi and nematodes in black pepper farms. The obtained results showed that the soil micro flora community was quite diverse. The average total density microorganism in Duc Co district, Chu Prong district and Chu Se district was of 8.41x10<sup>6</sup> CFU/g, 6.76x10<sup>6</sup> CFU/g and 8.91x10<sup>6</sup> CFU/g, respectively. The density of average total beneficial microorganisms in Duc Co district, Chu Prong district and Chu Se district was of 20.03x10<sup>4</sup> CFU/g, 14.25x10<sup>4</sup> CFU/g and 17.62x10<sup>4</sup> CFU/g, respectively. The use of organic fertilizers is common to the farms where high microbial densities were recorded. The relationship between the density of beneficial &nbsp;microorganisms (Nitrogen fixing bacteria, Phosphorus soluble bacteria, Cellulose degradation bacteria) and the density of fungal pathogens and nematodes was negatively correlated with R = - 0.84 and R = - 0.81 respectively. The results of correlation analysis showed that the density of beneficial microorganisms in the soil and the incidence of diseases in orchards were negatively correlated (R = - 0.69). This study proposes that the correlation between the basic factor of soil ecological system with pathogenic fungi and nematodes plays a very important role in biological control and the sustainable production of black pepper.</p> Thi Huyen Nguyen, Anh Dzung Nguyen, Nguyen Quang Vinh ##submission.copyrightStatement## Tue, 28 Jul 2020 00:00:00 +0000 Droplet Digital PCR – A Superior Complementary Technique for SARS-CoV-2 Detection <p>Accurate and timely SARS-CoV-2 detection in suspected persons is crucial in the fight against its spread. Many techniques have been developed to meet up with the continuously growing demand, however some of these techniques lack the required accuracy, sensitivity and specificity. The current reference standard technique for SARS-CoV-2 detection is RT-PCR, but studies have shown that false-negative results are inevitable and data can be non-reproducible when samples and primers are not appropriately verified and validated. Droplet digital PCR (ddPCR) is a newly introduced technique that performs precise nucleic acid quantification. Researchers have evaluated the efficacy of ddPCR and the technique has shown promising results even in specimens with low viral load. ddPCR has shown increased accuracy, precision, sensitivity and specificity. Furthermore, it is less affected by annealing and amplification inhibitors. This suggests that ddPCR can be used as a complementary detection technique especially in convalescent cases.</p> I. M. Hussaini, S. Gide, B. Musa, M. A. Sulaiman, A. Usman ##submission.copyrightStatement## Sat, 01 Aug 2020 00:00:00 +0000