Asian Journal of Biology 2021-07-24T11:25:41+00:00 Asian Journal of Biology Open Journal Systems <p style="text-align: justify;">The aim of <strong>Asian Journal of Biology&nbsp;(ISSN: 2456-7124)</strong>&nbsp;is to publish high quality papers (<a href="/index.php/AJOB/general-guideline-for-authors">Click here for Types of paper</a>) with broad areas of Aerobiology, &nbsp;Agriculture, Anatomy, Astrobiology, Biochemistry, Bioengineering, Bioinformatics, Biomathematics or Mathematical Biology, Biomechanics, Biomedical research, Biophysics, Biotechnology, Building biology, Botany, Cell biology, Conservation Biology, Cryobiology, Developmental biology, Food biology, Ecology, Embryology, Entomology, Environmental Biology, Epidemiology, Ethology, Evolutionary Biology, Genetics, Herpetology, Histology, Ichthyology, Integrative biology, Limnology,&nbsp; Mammalogy, Marine Biology, Microbiology, Molecular Biology, Mycology, Neurobiology, Oceanography, Oncology, Ornithology, Population biology, Population ecology, Population genetics, Paleontology, Pathobiology or pathology, Parasitology, Pharmacology, Physiology, Psychobiology, Sociobiology, Structural biology, Virology and&nbsp; Zoology. This is a quality controlled, peer-reviewed, open access INTERNATIONAL journal.</p> Impact of Coenzyme Q10 on Hormonal Profile in Male Sprague-Dawley Rat Exposed to Sub-Chronic Concentrations of Cypermethrin 2021-07-06T04:34:17+00:00 Eme Efioanwan Orlu Adetutu Olubunmi Obulor <p><strong>Aim: </strong>This study was aimed at evaluating the impact of Coenzyme Q10 on hormonal profile in male Sprague-Dawley rat exposed to sub-chronic concentrations of Cypermethrin.</p> <p><strong>Experimental Design:</strong> A completely randomized experimental design using standard methods for analysis. Hormonal assay was carried out by Microplate Enzyme Immunoassay using their respective test kits. Including AccuBind<sup>TM</sup> Microplate EIA Test system from Monobind Inc. Lake Forest CA 92630 USA while statistical analysis was carried out using one-way Analysis of Variance (ANOVA); where significant differences were found, Pair-wise comparisons conducted with Tukey test using SPSS 20 software.</p> <p><strong>Location and Duration of Study:</strong> This study was carried out in the Department of Biology, Ignatius Ajuru University of Education Rumuolumeni, Port Harcourt, Rivers State, Nigeria. GPS 4<sup>o</sup>48<sup>ʹ</sup>14<sup>ʹʹ </sup>N 6<sup>o</sup>59<sup>ʹ</sup>12<sup>ʹʹ </sup>E. The study lasted for 28days.</p> <p><strong>Methodology:</strong> Thirty male Sprague-Dawley rats were randomly assigned to five groups, A-E(n=6/group).Group A was given cool clean water and standard rat pellet ad libitum. Group B,C and D were administered Cypermethrin @ 10mg/kg/bw,20mg/kg/bw,30mg/kg/bw respectively along with 10mg/kg/bw of coenzyme Q10 (CoQ10). Group E received 30mg/kg/bw of cypermethrin only without Coenzyme Q10. All animals were allowed access to cool clean water and standard rat pellet ad libitum. Bodyweight of the animals were taken twice a week and recorded in grams. Twenty-four hours before the animals were euthanized with ethyl ether inhalation, feed was withdrawn from the animals. Blood samples were collected by cardiac puncture between the hours of 7:00 and 9:00am into plain sample tubes according to the approved protocol of blood collection techniques. Analysis for the quantitative determination of all androgens was by Microplate Enzyme Immunoassay using the AccuBind<sup>TM</sup> Microplate EIA Test system from Monobind Inc. Lake Forest CA 92630 USA and expressed as their respective units. The animals were latter dissected and the vital organs harvested and weighed. The values were subjected to statistical analysis using SPSS software 20.</p> <p><strong>Results:</strong> Results showed that there was no significant difference between the bodyweight and organ weight of animals in the treatment group compared with the control. Also, treatment with Cypermethrin only in group E, reduced the level of all the androgens considered in exposed animals. However, with concurrent administration of coenzyme Q10, the production of all androgens especially the production of Follicle Stimulating Hormone, Luteinizing Hormone and Progesterone in groups B,C,D and Estrogen in group B were significantly (P=.05) increased to values higher than the control.</p> <p><strong>Conclusion:</strong> This elevation indicates the role of Coenzyme Q10 as an effective antioxidant to boost hormonal level production especially the level of androgens.</p> 2021-07-03T09:24:01+00:00 ##submission.copyrightStatement## Aero-microbiology of Selected Poultry Farms in Imo State, South Eastern, Nigeria 2021-07-10T10:47:32+00:00 J. E. Ekeleme J. C. Ozougwu V. O. Nwaugo N. C. Nwachukwu C. N. Okorie I. Nwachukwu D. M. Smith-Akor <p>The aero-microbiology of selected poultry farms in Imo State, South Eastern Nigeria was investigated by microbial analysis. Result revealed that in Owerri poultry farm, Pm<sub>2.5</sub> had a range of 8-36±1.4 ug/m<sup>3</sup>, PM<sub>10</sub> had a range of 19-55±1.4ug/m<sup>3</sup>, a temperature range of 34.1-37.8±4.2<sup>o</sup>C and a relative humidity of 63.3-81.6±0.0%, while poultry farm at Okigwe had Pm<sub>2.5</sub> range of 8±1.4-21±1.4ug/m<sup>3</sup>, PM<sub>10</sub> had a range of 20±1.4- 88±2.8ug/m<sup>3</sup>, a temperature range of 28.6±1.2-38.1±0.0<sup>o</sup>c, and a relative humidity of 63.4±0.0-87.0±1.4%. Total heterotrophic bacterial count (THBC), total coliform count (TCC), Total <em>Staphylococal</em> count(TSC) and total fungi count (TFC) examined showed that THBC group had the highest counts with a range of 1.2-20.8cfu/plate/mins along the distances at 15minutes, 2.7-21.4cfu/plate/mins at 30minutes and 3.5-21.8cfu/plate/mins at 45minutes. While in Okigwe, THBC which was also the highest had a range of 09-22.5 along the distances at 15minutes, 1.2-24.8cfu/plate/mins at 30minutes and 1.0-4.0cfu/plate/mins at 45 minutes. Bacteria isolated were <em>Staphylococcus aureus, Escherichia coli, Proteus</em> and <em>Streptococcus</em> species while the fungi isolated were <em>Aspergillus, Penicillium, Fusarium Mucor</em> and <em>Rhizopus</em> Species. Temperature, particulate matter and microbial counts were highest during the dry season, while relative humidity was highest during the rainy season respectively. It was observed that as time increased along the distances examined, microbial load also increased. The aero-microbiology of the farms studied showed that the air contained presence of bacteria and fungi of medical importance in both seasons, hence, there is the need to always ensure proper management and evaluation of poultry farms to avoid micro-organisms from exceeding their permissive limits.</p> 2021-07-07T00:00:00+00:00 ##submission.copyrightStatement## Biodiversity and Phylogenetic Relationship of Total Hydrocarbon Degrading Genes in Selected Bacteria Species 2021-07-24T11:25:41+00:00 R. B. Agbor S. P. Antai S. E. Ubi <p>Hydrocarbons which forms the bulk of soil and water pollutants in the Niger Delta region of Nigeria differs in their susceptibility to microbial attack and degradation. Considering the importance of hydrocarbons in the economy of any nation, a complete knowledge of the hydrocarbons degrading capabilities of associated microbial species becomes increasingly important and indispensable. The research was aimed at assessing the biodiversity and phylogenetic relationship of hydrocarbon degrading genes in selected bacteria species. Sequences of nucleotides and amino acids of hydrocarbons degrading genes in 12 species of bacteria such as <em>Pseudomonas aeruginosa </em>I &amp; II, <em>P. stutgeni</em>, <em>Thalassosqir spp. </em>I &amp; II, <em>Alcavorox spp., Arthrobacter spp., Martellela spp., P. taenensis, Aneuribacillus </em>species, <em>Rhodococcus spp</em>. and Uncultured bacteria, were retrieved from the National Center for Biotechnology Information (NCBI) and analysed for their variability in physicochemical properties, percentage identity and similarity, G-C content, secondary and tertiary structures, their biodiversity and their phylogenetic relationship using MEGA 6 soft-wares, GOR IV, Phyre, Genscan and SIB Protparam. The analysis showed great genetic diversity and polymorphism in the hydrocarbon degrading genes present in the bacteria species. The phylogenetic tree showed that the genes in most species had undergone mutation. <em>Martella spp</em> had the highest probability or chance of mutating with E-value of 7e-129. Only two of the bacteria species had low instability index indicating that majority of the bacteria species contains or possessed the unstable nature of the hydrocarbon degrading genes. The biodiversity of bacterial species and their genetic distance was ascertained.</p> 2021-07-24T00:00:00+00:00 ##submission.copyrightStatement##