Haematological and Histological Effects of Nanoplastics Released from Nonfood-grade Nonwoven Polyethene Bags on Mice

Sadia Islam Reya

Genetics and Molecular Biology Laboratory, Department of Zoology, Faculty of Biological Science, University of Rajshahi, Rajshahi-6205, Bangladesh.

Md. Shihab Uddin

Genetics and Molecular Biology Laboratory, Department of Zoology, Faculty of Biological Science, University of Rajshahi, Rajshahi-6205, Bangladesh.

Mst. Nishat Tasnim

Genetics and Molecular Biology Laboratory, Department of Zoology, Faculty of Biological Science, University of Rajshahi, Rajshahi-6205, Bangladesh.

Md. Anisuzzaman

Genetics and Molecular Biology Laboratory, Department of Zoology, Faculty of Biological Science, University of Rajshahi, Rajshahi-6205, Bangladesh.

Sumaiya Islam Titly

Genetics and Molecular Biology Laboratory, Department of Zoology, Faculty of Biological Science, University of Rajshahi, Rajshahi-6205, Bangladesh.

Moni Krishno Mohanta

Genetics and Molecular Biology Laboratory, Department of Zoology, Faculty of Biological Science, University of Rajshahi, Rajshahi-6205, Bangladesh.

Md Fazlul Haque *

Genetics and Molecular Biology Laboratory, Department of Zoology, Faculty of Biological Science, University of Rajshahi, Rajshahi-6205, Bangladesh.

*Author to whom correspondence should be addressed.


Abstract

Nonfood-grade nonwoven polyethene (NFWP) bags which are frequently used in microwave cooking or carrying hot foods have been reported for releasing large amount of nanoplastics. Hence, the present study was designed to investigate the harmful effects of nanoplastics released from NFWP bags on mice. NFWP bags were cut into small pieces which were then boiled in water for 5 minutes. Then the boiled water was used as nanoplastic-contaminated drinking water for treated mice for 50 days. Surprisingly, the body weights and organ weight were increased remarkably for treated mice than control mice. The total Red Blood Cell (RBC) count decreased remarkably while total WBC count increased significantly in treated mice than in control. The percentage of neutrophils decreased remarkably while percentage of Monocytes increased significantly in treated mice as compared with control. The consumption of nanoplastics caused histological damage to intestine, heart, lung, kidney and liver of treated mice. The layers of intestinal muscles in the villi of treated mice were disrupted and infiltrated with foam cells.  Destruction of alveoli and fibrosis was observed in the lungs of the treated mice. The heart muscles of treated mice were also disrupted and irregularly arranged with fibrosis. In the kidney of treated mouse, enhancement of renal spaces, shrinkage of glomeruli, eroded Bowman’s capsule, deleted and congested glomeruli along with blood vessel were found. The liver of the treated mice was affected by apoptosis, fibrosis, vacuole formation in hepatocyte, congested in hepatic tissue and dilation of blood vessel. Therefore, it can be concluded that consumption of nanoplastics released from hot NFWP bags has serious deleterious effect on haematopoiesis and tissue integrity of different organs of Mice.

Keywords: Nanoplastics, polythene bag, haematology, histology, mouse


How to Cite

Reya, Sadia Islam, Md. Shihab Uddin, Mst. Nishat Tasnim, Md. Anisuzzaman, Sumaiya Islam Titly, Moni Krishno Mohanta, and Md Fazlul Haque. 2024. “Haematological and Histological Effects of Nanoplastics Released from Nonfood-Grade Nonwoven Polyethene Bags on Mice”. Asian Journal of Biology 20 (10):22-33. https://doi.org/10.9734/ajob/2024/v20i10440.