Investigation of the Pectin Degradation Ability of Hominibacterium faecale Strain SF3T Isolated from Human Feces
Asian Journal of Biology,
Page 38-44
DOI:
10.9734/ajob/2022/v15i130232
Abstract
Pectin is one of the most important dietary fibers as a prebiotic to determine the composition of human gut microbiome. The study was designed to investigate the pectin degradation ability of Hominibacterium faecale strain SF3T, its isolated from human feces and cultivated with different concentration pectin under anaerobic conditions in vitro. The growth ratio of strain SF3T formed in batch culture was examined every 10 h during the 60-h incubation time using the spectrophotometer, high-performance liquid chromatography, and gas-chromatography. The pure culture of Hominibacterium faecale pectin degradation activity was present and commonly increases the growth value of strain SF3T after pectin fermentation. Regarding volatile fatty acids, acetate, propionate and butyrate levels rapidly raised after 30 h of incubation. The results suggest that pectin fermentation displays the greatest contribution for human health and confirm that pectin degradation leads to the production of acetate, butyrate and propionate.
Keywords:
- Pectin
- degradation
- volatile fatty acids
- prebiotic
How to Cite
Borhanudin, N., Li, F., Faiq, M. E., & Cheng, L. (2022). Investigation of the Pectin Degradation Ability of Hominibacterium faecale Strain SF3T Isolated from Human Feces. Asian Journal of Biology, 15(1), 38-44. https://doi.org/10.9734/ajob/2022/v15i130232
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Zoetendal EG, et al. The human small intestinal microbiota is driven by rapid uptake and conversion of simple carbohydrates. The ISME Journal. 2012;6(7):1415-142.
Abbott DW, Gilbert HJ, Boraston AB. The active site of oligogalacturonate lyase provides unique insights into cytoplasmic oligogalacturonate β-elimination. Journal of Biological Chemistry. 2010;285(50):39029-38.
Downs, D.M., Understanding microbial metabolism. Annu Rev Microbiol. 2006;60:533-59.
Elshahed MS, Miron A, Aprotosoaie AC, Farag MA. Pectin in diet: Interactions with the human microbiome, role in gut homeostasis, and nutrient-drug interactions. Carbohydrate Polymers. 2021;255:117388.
Flint HJ, Duncan SH, Scott KP, Louis P. Interactions and competition within the microbial community of the human colon: Links between diet and health. Environmental microbiology. 2007;9(5):1101-11.
Onumpai C, Kolida S, Bonnin E, Rastall RA. Microbial utilization and selectivity of pectin fractions with various structures. Applied and Environmental Microbiology. 2011;77(16):5747-54.
Blanco-Pérez F, Steigerwald H, Schülke S, Vieths S, Toda M, Scheurer S. The dietary fiber pectin: Health benefits and potential for the treatment of allergies by modulation of gut microbiota. Current Allergy and Asthma Reports. 2021;21(10):1-9.
Hugouvieux‐Cotte‐Pattat N, Condemine G, Shevchik VE. Bacterial pectate lyases, structural and functional diversity. Environmental Microbiology Reports. 2014;6(5):427-40.
Kim CC, Healey GR, Kelly WJ, Patchett ML, Jordens Z, Tannock GW, Sims IM, Bell TJ, Hedderley D, Henrissat B, Rosendale DI. Genomic insights from Monoglobus pectinilyticus: a pectin-degrading specialist bacterium in the human colon. The ISME Journal. 2019;13(6):1437-56.
Chung WS, Meijerink M, Zeuner B, Holck J, Louis P, Meyer AS, Wells JM, Flint HJ, Duncan SH. Prebiotic potential of pectin and pectic oligosaccharides to promote anti-inflammatory commensal bacteria in the human colon. FEMS Microbiology Ecology. 2017;93(11):fix127.
Krishnan S, Alden N, Lee K. Pathways and functions of gut microbiota metabolism impacting host physiology. Current Opinion in Biotechnology. 2015;36:137-45.
Vega NM. Experimental evolution reveals microbial traits for association with the host gut. PLoS Biology. 2019;17(2):e3000129.
Scanlan PD, Marchesi JR. Micro-eukaryotic diversity of the human distal gut microbiota: qualitative assessment using culture-dependent and-independent analysis of faeces. The ISME Journal. 2008;2(12):1183-93.
Wei Z, Ma S, Chen R, Wu W, Fan H, Dai L, Deng Y. Aminipila luticellarii sp. nov., an anaerobic bacterium isolated from the pit mud of strong aromatic Chinese liquor, and emended description of the genus Aminipila. International Journal of Systematic and Evolutionary Microbiology. 2021;71(10):004710.
Yarza P, et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nature Reviews Microbiology. 2014;12(9):635-645.
Munyenyembe K, Timmons C, Weiner AK, Katz LA, Yan Y. DAPI staining and DNA content estimation of nuclei in uncultivable microbial eukaryotes (Arcellinida and Ciliates). European Journal of Protistology. 2021;81:125840.
Chen Y, Jiang X, Xiao K, Shen N, Zeng RJ, Zhou Y. Enhanced volatile fatty acids (VFAs) production in a thermophilic fermenter with stepwise pH increase–Investigation on dissolved organic matter transformation and microbial community shift. Water Research. 2017;112:261-8.
Fernández R, Dinsdale RM, Guwy AJ, Premier GC. Critical analysis of methods for the measurement of volatile fatty acids. Critical Reviews in Environmental Science and Technology. 2016;46(3):209-34.
Wang X, Carvalho G, Reis MA, Oehmen A. Metabolic modeling of the substrate competition among multiple VFAs for PHA production by mixed microbial cultures. Journal of biotechnology. 2018;280:62- 9.
Gálvez-Martos JL, Greses S, Magdalena JA, Iribarren D, Tomás-Pejó E, González-Fernández C. Life cycle assessment of volatile fatty acids production from protein-and carbohydrate-rich organic wastes. Bioresource Technology. 2021;321:124528.
Humerez-Flores JN, Kyomugasho C, Gutiérrez-Ortiz AA, De Bie M, Panozzo A, Van Loey AM, Moldenaers P, Hendrickx ME. Production and molecular characterization of tailored citrus pectin-derived compounds. Food chemistry. 2022;367:130635.
Liu Y, Heath AL, Galland B, Rehrer N, Drummond L, Wu XY, Bell TJ, Lawley B, Sims IM, Tannock GW. Substrate use prioritization by a coculture of five species of gut bacteria fed mixtures of arabinoxylan, xyloglucan, β-glucan, and pectin. Applied and Environmental Microbiology. 2020;86(2):e01905-19.
Flint HJ, Scott KP, Duncan SH, Louis P, Forano E. Microbial degradation of complex carbohydrates in the gut. Gut microbes. 2012;3(4):289-306.
Lopez-Siles M, Khan TM, Duncan SH, Harmsen HJ, Garcia-Gil LJ, Flint HJ. Cultured representatives of two major phylogroups of human colonic Faecalibacterium prausnitzii can utilize pectin, uronic acids, and host-derived substrates for growth. Applied and Environmental Microbiology. 2012;78(2):420-8.
Zoetendal EG, et al. The human small intestinal microbiota is driven by rapid uptake and conversion of simple carbohydrates. The ISME Journal. 2012;6(7):1415-142.
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