Detection and Enumeration of Coliforms in Drinking Water Sources in the Selected Barangay in Santa Maria, Davao Occidental Philippines
Asian Journal of Biology,
Page 1-7
DOI:
10.9734/ajob/2022/v15i130227
Abstract
The study aimed to determine the presence of fecal and nonfecal coliforms in selected drinking water sources of Santa Maria, Davao Occidental. Three (3) barangays were purposively identified as sampling sites, namely: Barangay Pongpong, Barangay San Isidro, and Barangay San Roque, where spring water, Jetmatic, and manual water pump are the sources of drinking water.
The study was conducted at Sta Maria, Davao Occidental Philippines from August 2021 to December 2021.The water samples were collected aseptically from the identified reservoir in the drinking water of the selected barangay of Sta Maria, Davao Occidental. The collected samples were brought to the SPAMAST Microbiology Laboratory. One (1) mL of sample was plated aseptically in the coliform Petrifilm. The plated water samples were incubated at 35-37 degrees Celcius for 24 hours. Colonies in the Petrifilm were counted manually and recorded as colony-forming units (CFU).
Microbial analysis detected the presence of total coliforms on the drinking water reservoir from the spring water source from barangay Pongpong, manual water pump and spring water source from barangay San Isidro and the manual water pump from barangay San Roque. Meanwhile, noOccidental, Philippines. Generally, the drinking water sources of the three (3) barangays exceeded the national standard set by the Department of Environment and Natural Resources (DENR) and the World Health Organization (WHO). Periodic monitoring on the drinking water sources in the identified barangays is highly recommended.
Keywords:
- Drinking water
- microbiology
- coliform
- public safety
How to Cite
References
Staci G. The Potential Importance of Conservation, Restoration and Altered Management Practices for Water Quality in the Wabash River Watershed. 2013December 09-13, 2013.
Available:https://cfpub.epa.gov/si/si_publi c_comments.cfm
Schafer A. Sustainable Water for the Future: Water Recycling Versus Desalination; 2010.
Available:https://www.elsevier.com/books/ sustainable- water-for -the- future/escobar/978-0-444-53115-5
Asian Development Bank. Water Supply and Sanitation Sector Assessment, Strategy, and Road Map; 2013. Retrieved last May 13 2022.
Available:https://www.adb.org/sites/defaul t/files/institutional- document/33810/files/philippines-water- supply-sector-assessment.pdf
Senate Economic Planning. Turning the tide: Improving water resource management in the Philippines (PB-11- 03); 2011.
Available:http://www.senate.gov.ph/public ations/PB%20201108%20%20Turning%20the%20Tide.pdf.
Department of Health. Coliform bacteria and drinking water. Department of Health, United States; 2016.
Available:https://www.doh.wa.gov/Portals/ 1/Documents/Pubs/331-181.pdf.
Bird P, Bastin B, Klass N, Crowley E, Agin J, Goins D, Bakken H, Lingle C, Schumacher A, & Collaborators. Evaluation of the 3M™ Petrifilm™ Rapid E. coli/Coliform Count Plate for the Enumeration of E. coli and Coliforms: Collaborative Study, First Action: 2018.13. Journal of AOAC International. 2020; 103(2):513–522.
Available:https://doi.org/10.1093/jaocint/q sz013
Beloti, Vanerli, Souza, Juliana Aparecida de, Barros, Márcia de Aguiar Ferreira, Nero, Luís Augusto, Mattos, Marcos Rodrigues de, Gusmão, Viviane Vieira, & Moraes, Luciane Bilia de. Evaluation of Petrifilm™ EC and HS for total coliforms and Escherichia coli enumeration in water. Brazilian Journal of Microbiology 2003;34(4):301-304.
Available: https://doi.org/10.1590/S1517- 83822003000400002
Vail JH, Morgan RM, Merino CR, Gonzales FS, Miller R, Ram JL. Enumeration of waterborne Escherichia coli with Petrifilm plates: comparison to standard methods. Journal of Environmental Quality. 2003;32(1):368- 73.
Pearson AL, Roberts MC, Soge OO, Ivanova IC, Mayer JD, & Meschke JS. Utility of EC 3 MTM Petrifilm TM and sanitary surveys for source water assessment in Nyabushozi County, south- western Uganda; 2008.
Wohlsen T, Bates J, Vesey G, Robinson WA, & Katouli M. Evaluation of the methods for enumerating coliform bacteria from water samples using precise reference standards. Letters in Applied Microbiology. 2006;42(4):350– 356.
Available: https://doi.org/10.1111/j.1472- 765X.2006.01854.x
Chowdhury Z, Francia M. UNICEF Philippines. Two billion people lack safe drinking water, more than twice lack safe sanitation; 2017.Retrieved last May 2022.
Available:https://www.unicef.org/philippine s/press-releases/two-billion-people-lack- safe-drinking-water-more-twice-lack-safe- sanitation
Department of Environment and Natural Resources. DENR Administrative order no. 2016-08. Retrieved last May 13, 2022.
Available: https://emb.gov.ph/wp- content/uploads/2019/04/DAO-2016- 08_WATER-QUALITY-GUIDELINES- AND-GENERAL-EFFLUENT- STANDARDS.pdf
Enguito MR, Matunog VE, Bala JJ, Villantes YL. “Water Quality Assessment of Carangan Estero in Ozamiz City, Philippines.”; 2013.
Tayone JC. “Biological and Chemical Characteristics of Groundwater in a Rural Settlement Area of Davao Oriental.” American Scientific Research Journal for Engineering, Technology, and Sciences. 2015;14:94-99.
Besagas RL, Asoy AY, Ceniza MS, Leopoldo GD, Dael NT, and Del Rosario RM. “Upland and Coastal Freshwater Sources in Misamis Oriental, Philippines: A Comparison of Water Quality.” Mindanao Journal of Science and Technology. 2015:13:1
Available:http://ejournals.ph/form/cite.php?id=12568 (accessed September 11, 2018)
Tonog MN, Poblete MM. "Drinking Water Quality Assessment in Selected Barangays in Laoang, Northern Samar, Philippines," International Journal of Environmental Science and Development. 2015;6(1):29-33, 2015.
Satar SS, Dagoc RJ, Dagoc KM, Valdez SA, Veloso KR, Mag-aso AV. Microbial Water Quality Assessment in Lake Lanao Along Marawi City, Lanao Del Sur, Mindanao, Philippines. Int. J. Biosci. 2020;16(2):12-18.
Dabalos E, Diamante CC. Water Quality Assessment in Barangay Santo Niño Sibuco, Zamboanga Del Norte, Mindanao, Philippines. Ciência. 2020;39(1):111–118.
Available:www.wmsu.edu.ph/research_jo urnal
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