Impact of Coenzyme Q10 on Hormonal Profile in Male Sprague-Dawley Rat Exposed to Sub-Chronic Concentrations of Cypermethrin
Asian Journal of Biology,
Aim: This study was aimed at evaluating the impact of Coenzyme Q10 on hormonal profile in male Sprague-Dawley rat exposed to sub-chronic concentrations of Cypermethrin.
Experimental Design: A completely randomized experimental design using standard methods for analysis. Hormonal assay was carried out by Microplate Enzyme Immunoassay using their respective test kits. Including AccuBindTM Microplate EIA Test system from Monobind Inc. Lake Forest CA 92630 USA while statistical analysis was carried out using one-way Analysis of Variance (ANOVA); where significant differences were found, Pair-wise comparisons conducted with Tukey test using SPSS 20 software.
Location and Duration of Study: This study was carried out in the Department of Biology, Ignatius Ajuru University of Education Rumuolumeni, Port Harcourt, Rivers State, Nigeria. GPS 4o48ʹ14ʹʹ N 6o59ʹ12ʹʹ E. The study lasted for 28days.
Methodology: Thirty male Sprague-Dawley rats were randomly assigned to five groups, A-E(n=6/group).Group A was given cool clean water and standard rat pellet ad libitum. Group B,C and D were administered Cypermethrin @ 10mg/kg/bw,20mg/kg/bw,30mg/kg/bw respectively along with 10mg/kg/bw of coenzyme Q10 (CoQ10). Group E received 30mg/kg/bw of cypermethrin only without Coenzyme Q10. All animals were allowed access to cool clean water and standard rat pellet ad libitum. Bodyweight of the animals were taken twice a week and recorded in grams. Twenty-four hours before the animals were euthanized with ethyl ether inhalation, feed was withdrawn from the animals. Blood samples were collected by cardiac puncture between the hours of 7:00 and 9:00am into plain sample tubes according to the approved protocol of blood collection techniques. Analysis for the quantitative determination of all androgens was by Microplate Enzyme Immunoassay using the AccuBindTM Microplate EIA Test system from Monobind Inc. Lake Forest CA 92630 USA and expressed as their respective units. The animals were latter dissected and the vital organs harvested and weighed. The values were subjected to statistical analysis using SPSS software 20.
Results: Results showed that there was no significant difference between the bodyweight and organ weight of animals in the treatment group compared with the control. Also, treatment with Cypermethrin only in group E, reduced the level of all the androgens considered in exposed animals. However, with concurrent administration of coenzyme Q10, the production of all androgens especially the production of Follicle Stimulating Hormone, Luteinizing Hormone and Progesterone in groups B,C,D and Estrogen in group B were significantly (P=.05) increased to values higher than the control.
Conclusion: This elevation indicates the role of Coenzyme Q10 as an effective antioxidant to boost hormonal level production especially the level of androgens.
- free radicals
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Ankley GT, Johnson RD. Small fish models for identifying and assessing the effects of endocrine-disrupting chemicals. Institute for laboratory Animal Research Journal. 2004;45:469–483.
Carlsen E, Giwercman A, Keiding N, Skakkebaek NE. Evidence for decreasing quality of semen during past 50yrs. British Medical Journals. 1992;305(6854):609-13.
Obulor-Godwin AO, Orlu EE, Daka ER, Oruambo IF, Ebere N. Cypermethrin-Induced anti-fertility of the ameliorating effect of lycopene supplementation in female rats. Journal of Agriculture and Veterinary Science. 2018;11(3):57-63.
Fenske RA, Lu CS, Simcox NJ, Loewenherz C, Touchstone J, Moate TF, Allen EH, Kissel JC. Strategies for assessing children's organophosphorus pesticide exposures in agricultural communities. Journal of Exposure Analysis & Environmental Epidemiology. 2000;10:662-671.
Anway MD, Cupp AS, Uzumcu M, Skinner MK. Epigenetic transgenerational actions of endocrine disruptors and male fertility. Science. 2005;308:1466–1469.
Gray LE, Ostby J. Effects of pesticides and toxic substances on behavioral and morphological reproductive development: Endocrine versus non-endocrine mechanisms. Toxicology and Industrial Health. 1988;14:159–184.
Obulor AO, Orlu EE. Protective role of lycopene on hormonal profile and post testicular functions of male rat exposed to sublethal doses of Cypermethrin. Journal of Advance in Biology and Biotechnology. 2019;21(4):1-9.
Vinggaard AM, Nellemann C, Dalgaard M, Jorgensen EB, Andersen HR. Antiandrogenic effects in vitro and in vivo of the fungicide prochloraz. Toxicological Science. 2002;69:344–353
Bian Q, Xu LC, Wang SL, Xia YK, Tan LF, Chen JF, Song L, Chang HC, Wang XR. Study on the relationship between occupational fenvalerate exposure and spermatozoa DNA damage of pesticide factory workers. Occupational and Environmental Medicine. 2004;61:999–1005
Xia YK, Bian Q, Xu LC, Cheng SP, Song L, Liu JY, et al. Genotoxic effects on human spermatozoa among pesticide factory workers exposed to fenvalerate. Toxicology. 2004;203:49–60.
Xu DX, Zhu QX, Zheng LK, Wang QN, Shen HM, Deng LX. Exposure to acrylonitrile induced DNA strand breakage and sex chromosome aneuploidy in human spermatozoa. Mutation Research. 2003;537:93–100.
Saxena P, Saxena AK. (2010). Cypermethrin Induced Biochemical Alterations in the Blood of Albino Rats. Jordan Journal of Biological Sciences. 2010;3(3):111 – 114.
Bhushan B, Pande S, Saxena N, Saxena PN. Serum biochemical responses under stress of Cypermethrin in albino rat. Environmental and Experimental Biology. 2013;11:81-89.
Li YF, Pan C, Hu JX, Li J, Xu LC. Effects of cypermethrin on male reproductive system in adult rat. Biomedical and Environmental Sciences. 2012;26(3):201-8.
Gray LE, Jr, Wilson VS, Stoker T, Lambright C, Furr J, Noriega N, Howdeshell K, Ankley GT, Guillette L. Adverse effects of environmental antiandrogens and androgens on reproductive development in mammals. International Journal of Andrology. 2006;29:96– 104.
Iboroma M, Orlu EE, Ebere N, Obulor AO. Androgenic and Antioxidant Activity of stelleria media on Rat following sub-chronic exposure to Dichlorvos. Journal of Pharmacy and Biological Sciences. 2018;13:38-46.
Sun H, Xu LC, Chen JF, Song L, Wang XR. Effect of bisphenol A, tetrachlorobisphenol A and pentachlorophenol on the transcriptional activities of androgen receptor-mediated reporter gene. Food Chemistry and Toxicology. 2006;44:1916–1921.
Elbetieha A, Daas SI, Khamas W, Darmani H. Evaluation of the toxic potentials of Cypermethrin pesticide on some reproductive and fertility parameters in the male rats. Archives of Environmental Contamination Toxicology. 2001;41:522-528.
Obulor AO, Orlu EE. Evaluation of the therapeutic effect of Lycopene on Cypermethrin-induced reproductive toxicity in rats. Journal of Environmental Science, Toxicology and Food Technology. 2018b;12(3):10-16.
Nasr S, Abd El-Moneim A, Moghazy A, Badawi MM. Evaluation of an Antioxidant Containing Drug Action against Pyrethroid Toxicity. Current Science International. 2015;4(3):455-466.
Sharma P, Singh R. Protective role of curcumin on lindane induced reproductive toxicity in male Wistar rats. Bulletin of Environmental Contamination and Toxicology. 2010;84:378–84
Abou El-Magd SA, Laila ME, Sabik, Shoukry A. Pyrethroid toxic effects on some hormonal profile and biochemical markers among workers in pyrethroid insecticidescompany. Life Science Journal. 2011;8(1):311-322.
Gajraj SC, Joshi. Assessment of reproductive toxicity induced by Deltamethrin in male albino rats. Iran J Toxicol. 2009;2(3):194–202.
Bretveld RW, Thomas CM, Scheepers PT, Zielhuis GA, Roeleveld N. Pesticide exposure: the hormonal function of the female reproductive system disrupted. Reproductive Biologyand Endocrinology. 2006;4(30).
Showell MG, Brown J, Yazdani A, Stankiewicz MT, Hart RJ. Antioxidants for male subfertility. Cochrane Database System. Review. 2011;1.
Kucharska J, Braunova ZO, Uliena I. Deficit of coenzyme Q10 in heart and liver mitochondria of rats with streptozotocin-induced diabetes. Physiology Research. 2000;49:411-418.
Mongthuong TT, Mitchell TM, kennedy DT, Giles GT. Role of coenzyme Q10 in chronic heart failure, angina and hypertention. Pharmacotheraphy. 2001;21:797-806.
Bookstaver DA, Burkhalter NA, Hatzigeorgiou C. Effect of coenzyme Q10 supplementation on statin-induced myalgias. American Journal of Cardiology. 2012;110(4):526–529.
Tietz NM. Fundamental of Clinical Chemistry. 2nd Ed. Philadelphia. 1994;692.
Xu LC, Liu L, Ren XM. Evaluation of androgen receptor transcriptional activities of some pesticides in vitro. Toxicology. 2008;14:243(1-2):59-65.
Orlu EE. Deltamethrin-induced Alterations in Sperm Morphology and Spermatogenesis Impairment in Adult Sprague-Dawley Rats. Research Journal of Applied Sciences, Engineering and Technology. 2014;(11):2324-2331.
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