Phytochemical Comparison of Stem and Leaves of Cupressus sempervirens L. from Cuba

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Eva Salas
Chavelys López
Bárbara González
Flavio José Hernández Bonilla
Jhunior Abrahan Marcía Fuentes


The Cupressus sempervirens Linnaeus (Cypress) species, belonging to the botanical family of the Cupressaceae, is an arboreal species native to the eastern Mediterranean and is of great importance for presenting metabolites with great therapeutic potential. In Cuba, it is uncommon, and this is the reason why this research took place to compare the chemical characterization of the stem and leaves of the ¨Ciprés¨ of Cuban origin. The present research is the first one in Cuba for the species which underwent a pharmacognostic study of the crude drug and the extracts of the plant parts. To be carried out, quality parameters of the leaves and stem were evaluated where the results obtained met with the specifications described in the official monographs. The Maceration Method was applied using 95% ethanol for the extraction of its chemical constituents. These were qualitatively determined by the Phytochemical Screening Techniques; expressing with greater incidence essential oils, fats, alkaloids, triterpenoids and steroids, resins, reducing sugars, saponins, flavonoids, phenolic compounds, mucilages, and bitter principles. High-Performance Liquid Chromatography suggests that the compounds that showed retention time on the stem were 15.2 and 15.9 minutes and the retention times on leaves were 15.2 and 16.0 minutes.

Cupressus sempervirens, chemical composition, cypress, mediterranean.

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How to Cite
Salas, E., López, C., González, B., Bonilla, F. J. H., & Fuentes, J. A. M. (2020). Phytochemical Comparison of Stem and Leaves of Cupressus sempervirens L. from Cuba. Asian Journal of Biology, 10(1), 48-55.
Original Research Article


Marcía Fuentes J. Montero Fernández I, Zumbado H, Lozano Sánchez J, Santos Alemán R, Navarro Alarcón M, Bórras Linares I, Saravia S. Quantification of Bioactive Molecules, Minerals and Bromatological Analysis in Carao (Cassia grandis). Journal of Agricultural Science. 2020;12 (3);88-94.

Scherer R, Rybka ACP, Ballus CA, Meinhart AD, Filho JT, Godoy HT. Validation of a HPLC method for simultaneous determination of main organic acids in fruits and juices. Food Chemistry. 2012;135:150-154.

Ayala JF, Vega V, Rosas C, Palafox CH, Villa C, Wasim M, Dávila JE, González GA. Agro-industrial potential of exotic fruit byproducts as a source of food additives. Food Research International. 2011;44: 1866-1874.

Montero Fernández I, Saravia S, Perpetua V, Santos R, Marcía Fuentes J, Filho A. Chemical characterization of seeds of Amazon fruits as nutritional contribution with functional medicinal potential. African Journal of Pharmacy and Pharmacology. 2020;4(14):67-76.

Roig JT. Plantas medicinales, aromáticas o venenosas de Cuba. Ed. Científico-Técnica. La Habana, Cuba; 1988.

Tapondjou AL, Adler C, Fontem DA, Bouda H, Reichmuth C. Bioactivities of cymol and essential oils of Cupressus sempervirens and Eucalyptus saligna against Sitophiluszeamais motschulsky and Tribolium confusum du Val. J. Stored Prod. Res. 2005;41:91-102

Mazari K, Bendimerad N, Bekhechi C, Fernández X. Chemical composition and antimicrobial activity of essential oils isolated from Algerian Juniperus phoenicea L. and Cupressus sempervirens L. Journal of Medicinal Plants Research. 2010;4(10):959-964.

Elansary H, Salem M, Ashmawy NA, Yacout M. Chemical composition, antibacterial and antioxidant activities of leaves essential oils from Syzygium cumini L., Cupressus sempervirens L. and Lantana camara L. from Egypt. Journal of Agricultural Science. 2012;4(10):144-152.

Miranda M, Cuéllar A. Farmacognosia y Productos Naturales. Libro de texto. Edit. Félix Varela. La Habana, Cuba; 2001.

Arango GJ. Alcaloides y compuestos nitrogenados. Tesis Doctorado en Química, Facultad de Química Farmacéutica, Universidad de Antioquia, Medellín, Colombia; 2008.

Hih-Hsien C. Flavonoids, coumarins and acridone alkaloids from the root bark of Citrus limonia. Phytochemistry. 1990;29: 351-353.

Islam SKN, Gray AY, Waterman PG, Ahasan M. Screening of eight alkaloids and ten flavonoids isolated from four species of the genus Boronia (Rutaceae) for antimicrobial activities against seventeen clinical microbial strains. Phytotherapy, Res. 2002;(39):672-674.

Khokhar S, Apenten RKO. Iron binding characteristics of phenolic compounds: some tentative-structure activity relations. Food Chem. 2003;(81):133-140.

Montes de Oca Y. Compuestos bíoactivos con interés farmacéutico. Tesis Máster en Química Farmacéutica, Instituto de Farmacia y Alimentos, Universidad de La Habana, Cuba; 2015.

Harborne JB, Mabry TJ, Mabry H. The Flavonoids. Chapman and Hall. 1975;47-61.

Janet AM, Duthie GFlavonois in foods. Chemistry, Biochemistry and applications. CRC press: Boca Raton. 2006;219-262.

Al-Snafi A. Medical importance of Cupressus sempervirens - A review. IOSR Journal of Pharmacy. 2016;6(2):66-76.

De Luca A, Graziani E, Anticoli S, Simeoni S, Terzano C, Mannino F. Respiratory allergy to Cupressus sempervirens in Rome. Allergol Immunopathol. 1997;25(5): 229-232.

Koriem K. Lead toxicity and the protective role of Cupressus sempervirens seeds growing in Egypt. Rev Latinoamer Quím; 2009;37(3):230-242.