Main Article Content
Oligosaccharins: oligogalacturonic, and chitooligosaccharides are known as molecular signals to induce and regulate various genes in plants. This study was conducted to deternine the effects of chitooligosaccharide on bud formula and growth of Piper nigrum in both in vitro and ex vitro. The results showed that sterilize Piper nigrum shoots with 30% sodium hypochlorite at 10 min was the most suitable condition; appropriate culture media for bud formulation was Murashige and Skoog (MS) media supplemented 30 g/L saccharose, 7,5 g/L agar, 3 mg/L N6 – benzyl adenine (BA), culture media for growth of plantlet shoot was MS media supplemented 30 g/L sacharose, 7,5 g/L agar, 1 g/L NAA, 2 mg/L IBA and 45 ppm chitooligosaccharide. Supplementation of chitooligosaccharide at concentration of 45 ppm was optimal for the growth of Piper nigrum plantlets both in vitro and ex vitro. Present study indicated that chitooligosaccharide strongly promote the growth of Piper nigrum and recommend concentration for both in vitro and ex vitro is 45 ppm.
Limpanavech P, Chaiyasuta S, Vongpromek R, Pichyangkura R, Khunwasi C, Chadchawan S, Lotrakul P. Chitosan effects on floral production, gene expression, and anatomical changes in the Dendrobium orchid. Sci. Hortic. 2008;116: 65-72.
Hadwiger LA, Klosterman SJ, Choi JJ. The mode of action of chitosan and its oligomers in inducing plant promotors and developing disease resistant in plants. Advances in Chitin Science. 2002;452-457.
Li CF, Wu JC. Chitosan benefit cultivation of vegetables. Advances in Chitin Science. 1998;448-451.
Chandrkracchang S. The application of chitin and chitosan in agriculture in Thailand. Advances in Chitin Science. 2002;458-462.
Dzung NA. Chitosan and their derivatives as prospective biosubstances for the developing sustainable eco-agriculture. Advances in Chitin Science and Technology. 2007;453-459.
Dzung NA, Thang NT. Effects of oligoglucosamine prepared by enzyme degradation on the growth of soy bean. Advances in Chitin Science. 2002;463-467.
Dzung NA, Thang NT. Effects of oligoglucosamine on the growth and development of peanut (Arachis hypogea L). The 6th Asian-Pacific on Chitin, Chitosan Symposium. 2004;981-986.
Chibu H, Sibayyama H. Effect of chitosan application on the growth of several crops. Chitin and Chitosan in Life Science. FAO. 2000;235-239.
Dzung NA, Khanh VTP, Dung TT. Research on impact of chitoologosaccharide on biophysical characteristic, growth and development and drought resistance of coffee. Carbohydr. Polym. 2010;84(2):751-755.
Dzung NA, Khanh VTP, Ngoc NTB. Study on effects of chitosan, chiooligosaccharide and their derivatives on the growth inhibition of pathogenous fungi Phytopthora capsici and application for biocontrol black pepper. Advances in Chitin Science. 2006;505-507.
Uddin A, Hashimoto F, Shimizu K, Sakata Y. Monosaccharides and chitosan sensing in bud growth and petal pigmentation in Eustoma grandiflorum (Raf.) Shinn. Sci. Hortic. 2004;100(1-4):127-138.
Dzung NA. Application of chitin, chitosan and their derivatives for agriculture in Vietnam. J. Chitin Chitosan. 2005;10(3): 109-113.
Akter M, Julakha, Rahman M, Abdullah G, Dipali R, Surovy, MZ, Rahman M, Islam MT. Chitosan and plant probiotics application enhance growth and yield of strawberry. ISBAB. 2017;11:9-18.
Luan L, Ha V, Nagasawa N, Kume T, Yoshii F, Nakanishi T. Biological effect of irradiated chitosan on plants in vitro. Biotechnol. Appl. Biochem. 2005;41(1):49-57.
Luan LQ, Nagasawa N, Tamada M, Nakanishi TM. Enhancement of plant growth activity of irradiated chitosan by molecular weight fractionation. Radioisotopes. 2006;55(1):21–27.
Nge K, Nwe N, Chandrkrachang S, Stevens W. Chitosan as a growth stimulator in orchid tissue culture. Plant Science. 2006;170(6):1185-1190.
Bittelli M, Flury M, Campbell G, Nichols E. Reduction of transpiration through foliar application of chitosan. Agric. For. Meteorol. 2001;107(3):167-175.
NEDSPICE. Black pepper crop report. ESA Conference 1 June 2017 Bordeaux, France; 2018.
Phan TT, Le DN, Burny H, Lebailly. Economic analysis of perennial crop systems in Dak Lak Province, Vietnam. Sustainability. 2019;11(1).
Dzung NA, Sy ND. Study on chitooligosaccharide application for in vitro tissue culture of sweet potato (Ipomea batatas L.). 2010;15(3):141-146.
Dzung NA, Thuoc V. Study on effects of chitiooligosaccharide on the growth development and disease resistance of rice. Advances in Chitin Science and Technology. 2006;381-383.