Mineral Status and Lupine Yield Responses to Ascorbic Acid Spraying and Irrigation by Diluted Sea Water

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M. M. Hussein
A. Abd El-Khader
S. Y. El-Faham


A pot experiment was conducted in the greenhouse of the National Research Centre at Dokki, Cairo Egypt during 2010 -2011 winter seasons to evaluate the effect of different salt stress degrees on the growth and yield characters. The salinity treatments were: Irrigation by three concentrations of diluted seawater (2000, 4000 and 6000 ppm) more than the control treatment (irrigated by tap water 250 ppm) and spraying ascorbic acid (AsA) with two concentrations (100, and 200 ppm). Salinity depressed the pods, straw, straw+ pods and seeds weight relative to the control plants but the depression of these traits showed its maximum values when plants subjected to the higher level of salinity (6000 ppm) markedly more than that with the other two levels of salinity. Slight differences in the mentioned characters of plants irrigated by solution contained 2000 or 4000 ppm. Gradual depressions in pods/straw, seeds/pods and seeds/straw ratios were detected with the increase in salt concentration in water of irrigation. Ascorbic acid application led to increase the pod, straw, total and seeds yields. The high increment in pods weight and seeds to straw ratio wereshown by using 100 ppm ascorbic acid but the increment in straw, totalseeds weight and seeds/pods and pods/straw ratios.Furthermore, the absorption rate of N, K, P, Ca, and Mg ions from the growth medium significantly inhibited as a result of treatment with diluted sea water. Meanwhile, significant increases in the uptake of these ions were obtained in response to ascorbic acid application.

Lupine (Lupinus termis L), salinity-ascorbic acid, yield-mineral status

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Hussein, M., El-Khader, A., & El-Faham, S. (2019). Mineral Status and Lupine Yield Responses to Ascorbic Acid Spraying and Irrigation by Diluted Sea Water. Asian Journal of Biology, 8(2), 1-13. https://doi.org/10.9734/ajob/2019/v8i230059
Original Research Article


Takamatsu S, Saito K, Sekine T, Ohmiya S, Kubo H. Glycosidic alkaloids from Lupinus hirsutus. Phytochem. 1990;29:3923–6.

Tackholm V. Student flora of Egypt.2nd Ed. Cairo Univ.Press, Cairo, Egypt. 1974;224.

Prusinski J. White lupine (Lupinus termis L). Nutritional and health values in human nutrition. Review Czech. Food Sci. 2017;32(2):95-105.

FAO. The state of food and Agriculture: Climatic changes and food security, FAO, Roma; 2016.

Hussein MM, Youssef RA, Nesreen H. Abo-Bakr. Influences of potassium foliar fertilization and irrigation by diluted seawater on growth and some chemical constituents of cotton. IJSR. 2014;3(11):3127-3134.

Hussein MM, El-Faham SY. Chlorophyll, carotenoids pigments and growth of three onion cultivars as affected by saline water irrigation. Egypt. J. Agron. 2018;40(3):285-296.

Hussein MM, Abou-Baker NH. The contribution of nano-zinc to alleviate salinity stress on cotton plants. R. Soc. Opensci. 2018;5:1-11.171809.

Costa JH, Jolivet Y, Sauder MP, Orelleno EG, Lima MG, Dizengremel P, Melo DF. Alternative oxidase regulation in root of Vignaunguiculata cultivars differing in drought/salt tolerance. J. Plant Physiology. 2007;184:718-727.

Hussein MM, Safi-Naz S. Zaki. Influence of water stress on growth and photosynthetic pigments of some Fenugreek Varieties. Original Articles. Journal of Applied Sciences Research. 2013;9(8):5238-5245.

Hussein MM, Mehana HM, Zaki S, Abd El-Hadi N. Influences of salt-stress and foliar fertilizers on growth chlorophyll and carotenoids of jojoba plants. Middle East Journal of Agric. 2014c; 3(2):221-226.

Hussein MM, Abd El Hady NF. Growth and photosynthetic pigments responses of durum wheat varieties to irrigation by diluted sea water. International Journal of Science and Research (IJSR). 2014a;3(12):1656-1663.

Hussein MM, Bekheta MA, Safi-naz S. Zaki. Influence of uniconazole on growth characters, photosynthetic pigments, total carbohydrates and total soluble sugars of Hordium vulgare L plants grown under salinity stress. Intr. J. of Sci. and Res. (IJSR). 2014b;11(3):2208-2214.

Hussaein MM, Alva AS. Effect of zinc and ascorbic acid application on the growth and photosynthetic pigments of millet plants grown under different salinity. Agric. Sci. 2014;5:1263-1270.

Brugnoli E, Lauteri M. Effects of salinity on stomatal conductance, photosynthetic capacity and carbon iotope discrimination of salt-tolerant (Gossypium hirsutum L.) and ssalt- sensitive (Phaseolus vulgaris L.) C(3) Non-Halophytes. Plant Physiol. 1991;95(2):628-635.

Ashraf M, Apse MP, Blumwald E. Engineering salt tolerance in plants. Curropin Biotechol. 2002;13:145-150.

Beltagy MS. Exogenous ascorbic acid (vitamin C) induced anabolic changes for salt tolerance in chick pea (Cicer arietinum L.) plants. African Journal of Plant Science. 2008;2(10).

Orabi SH, Hussein MM, Safi-naz S. Zaki, Faida A. Sharara. Influence of hydrogen peroxide on growth, yield and biochemical constituents of canola plants grown under different irrigation intervals. Current Science International. 2018;3(7):407-418.

Orabi SH, Hussein MM, Abd El-Motty EZ, El-Faham SY. Effect of Alpha-tochophyrol and glutamic acid on total phenols, antioxidant activity, yield and fruit properties of mango trees. Middle East G. of Applied Sci. 2018;8(4):1229-1239.

Bernstein N, Shoresh M, Xu Y, Huang B. Involvement of the plant antioxidative response in the differential growth sensitivity tosalinity of leaves vs roots during cell development. Original Research Article. Free Radical Biology and Medicine. 20107:49:1161-1171.

Hafsi C, Romero-Puertas MC, Gupta DK, Del Río LA, Sandalio LM, Abdelly C. Moderate salinity enhances the antioxidative response in the halophyte Hordeumm aritimum L. under potassium deficiency. Original Research Article. Environmental and Experimental Botany. 2010;69(2):129-136.

Agati G, Biricolti S, Guidi L, Ferrini F, Fini A, Tattini M. The biosynthesis of flavonoids is enhanced similarly by UV radiation and root zone salinity in L. vulgare leaves. Original Research Article. Journal of Plant Physiology. 2011;168(3):204-212.

Talaat NB. Physiological studies on the effect of salinity, ascorbic acid and putresine of sweet pepper plant. Ph.D. Thesis, Fac. of Agric., Cairo Univ., Cairo, Egypt; 2003.

Maggio G, Raimondi A, Martino A, Pascale S. De. Effects of ascorbic acid applications on eggplants response to salinity. ISHS Acta Horticulturae, 747: VIII International symposium on Protected Cultivation in Mild Winter Climates: Advances in Soil and Soilless Cultivation under Protected Environment; 2006.

Abd El-Baky HH, Hussein MM, Baroty GS. Algal extraction improve antioxidants defense Abilities and salt tolerance of wheat plant irrigated with sea water. Electronic Journal of Environmental Agriculture and Food Chemistry. 2008;7(4):281–2832.

Cottenie A, Verlo M, Kiekeus L, Velghe G, Camerlynck R. Chemical Analysis of plantsand soils. Laboratory of Analytical and Agrochemistry State University, Ghent- Belgium; 1982.

Snedecor GW, Cochran WG. Statistical methods 8th Ed. Iowa State Univ. Iowa, USA; 1980.

Hussein MM, Shaaban MM, El-Saadym AM. Response of cowpea plants grown under salinity stress to PK-foliar application. American J. of Plant Physiol. 2008;3(2):81–88.

Overland M, Sørensen M, Sorebakken T, Penn M, Krogdahl A, Skrede A. Pea protein concentrate substituting fish meal or soybean meal in diets for Atlantic salmon (Salmosalar) - Effect on growth performance, nutrient digestibility, carcass composition, guthealth, and physical feed quality. Aquaculture. 2009;288(3-4):305–311.

Kong-ngem K, Dadurang S, Wong Kham C, Bunnag S, Koslttrakum M. Three seedlings. Sci. Asia, 2008;31:403-498.

Khalil, SE, Hussein MM, da Silva JT. Roles of antitranspirants in improving growth and water relations of Jatropha curcas L. grown under water stress conditions. Plant Stress. 2012; 6(1):49-54.

Hussein MM, Abo lilla BH, Mohamed S, El-Liethy S. Effect of irrigation by salt water using Stroganov solution on anatomical structure of jatropha plants. Australian J. of Basic and Applied Sci. 2012;8(1):491-496.

Khan W, Prithiviraj B, Smith DL. Nod factor [Nod Bj V (C18:1, MeFuc)] and lumichrome enhance photosynthesis and growth of corn and soybean. Journal of Physiology. 2008;165(13): 1342-1351.

Hayat S, Hasan, SA, Yusuf M, Hayat Q, Ahmad A. Effect of 28- homobrassinolide on photosynthesis, fluorescence and antioxidant system in the presence or absence of salinity and temperature in Vignaradiata. Environmental and Experimental Botany, In Press, Corrected Proof; 2010.

Shakirova FM, Sakhabutdinova AR, Bezrukova MV, Fatkhutdinova RA, Fatkhutdinova DR. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity Plant Science. 2003;164(3):317-322.

Bakheta MA, Hussein MM. Uniconazole effect on endogenous hormones, proteins and proline contents of barley plants (Hordium vulgare) under salinity stress (Nacl). Bioscience J. 2014;6(1):39-44.

Hussein MM, Oraby SH. Growth and antioxidant enzymes activity in onion plants as affected by thiamine and salinity. Plant Nutrition Management under Water Stress Conditions. 17th Inter. Symposium of CIEC. 2008 NRC, Cairo. 2008;II:260-278.

Misra N, Saxena P. Effect of salicylic acid on prolinemetabolism in lentil grown under salinity stress. Plant Science. 2009;17(3):181-189.

Tavallali V, Rahemi M, Maftoun M, Panahi B, Karimi S, Ramezanian A, Vaezpour M. Zinc influence and salt stress on photosynthesis, water relations, and carbonic anhydrase activity in pistachio. Scientia Horticulturae. 2009;123(2):272-279.

Hussein MM, EL-Gereadly HM, EL-Desuki M. Role of puterscine in resistance to salinity of pea plants (Pisum sativum L.) Journal of Applied Science Research. 2006;2(9):598-604.

Daei G, Ardekani MR, Rejali F, Teimuri S, Miransari M. Alleviation of salinity stress on wheat yield, yield componen. Journal of Plant Physiology. 2009;166(6):617-625.

Hussein MM, El-Saady AM, Nesreen H, Abo-Talb. Castor bean plants response to phosphorus sources under irrigation by diluted seawater. Intr. J. of Chem. Tech. Res. 2015;8(9):261-271.

Hussein MM, El-Saady AM, Nesreen H. Abo-Talb. Castor bean plants response to phosphorus sources under irrigation by diluted seawater. Intr. J. of Chem. Tech. Res. 2015;8(9):261-271.

Li R, Shi F, Fukuda K. Interactive effects of various salt and alkali stresses on growth, organic solutes and cation accumulation in a halophyte Spartina alterniflora (Poaceae). Environmental and Experimental Botany. 2010;68(1):66-74.

Ashraf M, Ahmad S. Influence of sodium chloride on ions accumulation, yield components and fiber characteristics in salt-tolerant and salt-sensitive lines of cotton (Gossypium hirsutum L.). Field Crops Research. 2000;66(2):115-127.

Kinraide TB. Interactions among Ca2+, Na+ and K+ in salinitytoxicity: Quantitative resolution of multiple toxic and ameliorative effects. Journal Experimental Botany. 1999;50:1495-1505.

Fernandes FM, Arrabaça M, Carvalho LMM. Sucrose metabolism in Lupinusalbus L. under salt stress.Biologia Plantarum. 2004;48(2):317-319.

Keutgen AJ, Pawelzik E. Quality and nutritional value of strawberry fruit under term salt stress. Food Chemistry. 2008;107(4):1413-1420.

Athar HR, Khan A, Ashraf M. Exogenously applied ascorbicacid alleviates salt- induced oxidative stress in wheat. Environmental and Experimental Botany. 2008;63(1-3):224-231.

Salah IB, Albacete A, Andújar CM, Haouala R, Labidi N, Zribi F, Martinez V, Pérez-Alfocea F, Abdelly C. Response of nitrogen fixation in relation to nodule carbohydrate metabolism inMedicago ciliaris lines subjected to salt stress. Journal of Plant Physiology. 2009;166(5):477-488.

Shetewie SA. Improving growth and yield of salt stressed soybeanby exogenous application of asmonic acid and ascorbin. Intr. J. Agric. and Biol. 2007;9(3):473–478.

Foyer CH. Ascorbic acid: Alscher RG, Hess JL. eds. Antioxidants in Higher Plants Boca Raton CRC Press. 199331-58.

Nour TA, Hussein MM, Lotfi A. Nitrogen forms and chloride salinity and its effects on growth and chemical status of wheat seedling. Egypt. J. Agron. 1989;14(1).

Abdel Rasoul M, Hussein MM, Ashoub MA. Effect of some growth regulators on the concentration of organic and amino acids in maize seedling under different levels of salinity 1- The concentration of organic acids. Ain Shams, Agric. Res. Bull., No.1330. 1980;10.

Mohamed AA, Eichler Lobermann B, Schnug E. Response of crops to salinity under Egyptian conditions: a review. Landbauforsch Volk. 2007;2(57):119-125.

Hanafy Ahmed AH, Higazy MA, El-Shafey YH, Moussa SF. Effect of salinity, silicon and proline on the growth, yield and chemical composition of wheat plant. Proceedings of the 2nd Congress Recent Technology Agriculture, Cairo University. 2002;965-978.

Tarraf SA, Kmge D, Balbaa LK. The response of vegetative growth, essential oil of lemongrass (Cymbopogon citratus Hort.) to foliar application of ascorbic acid nicotinamide and some micronutrients. Arab Universities Journal of Agricultural Sciences. 1999;7(1):247-259.

Singh KN, Chatrath R. Salinity tolerance. In: Reynolds M.P., Monasterio JIO, Mc Nab A, editors. Application of Physiology in Wheat Breeding. CIMMYT; Mexico, DF: 2001;101–110.

Neveen Shawky. NBET. Physiological studies on the effect of salinity, ascorbic acid and putrescine on sweet pepper plant. Ph.D. Thesis, Dept. of Agric. Bot., Faculty of Agric. Univ. of Cairo; 2003.

Wolucka BA, Goossens A, Lnze D. Methyl jasmonate stimulates the novo biosynthesis of Vitamin C in plant cell suspense’s. J. of Exp. Bot. 2005;56(419):2527-2538.

M’rah S, Ouerghi Z, Eymery F, Rey P, Hajji M, Grignon C, Lachaâl M. Efficiency of biochemical protection against toxic effects of accumulated salt differentiates Thellungiellahalophila from Arabidopsis thaliana. Journal of Plant Physiology. 2007;164(4):375-384.

Xu W, Shi W, Ueda A, Takabe T. Mechanisms of salttolerance in transgenic Arabidopsis thaliana carrying a peroxisomalscorbate peroxidase gene from barley. Pedosphere. 2008;18: 486-495.

Zabalza A, Gálvez L, Marino D, Royuela M, Arrese-Igor C, González EM. The application of ascorbate or its immediate precursor, galactono-1,4-lactone, does not affect the response of nitrogen-fixing pea nodules to water stress. Journal of Plant Physiology. 2008;165:805-812.

Lu Z, Liu D, Liu S. Two rice cytosolic ascorbate peroxidases differentially improve salt tolerance in transgenic Arabidopsis. Plant Cell Reports. 2007;26:1909–1917.