Nephroprotective Effect of Cissampelos owariensis Extract on Renal Histomorphology of Wistar Rats during Exposure to Carbon Tetrachloride- induced Nephropathy

Main Article Content

Dayo Rotimi Omotoso
Olayinka Simbiat Lawal
Oluwasegun Davies Olatomide
Itohan Grace Okojie

Abstract

To assess nephroprotective potency of methanolic extract of C. owariensis on renal histomorphology of Wistar rats during exposure to nephropathic activity of CCl4.

Twenty eight (28) albino Wistar rats divided into four groups which include normal control group administered with vehicles -distilled water (1 ml/kg b.w.) and olive oil (3 ml/kg b.w.), experimental control group administered with CCl4 (3 ml/kg b.w.) twice a week, first treatment group administered with CCl4 (3 ml/kg b.w.) twice a week + methanolic extract of C. owariensis (100 mg/kg b.w.) daily and second treatment group administered with CCl4 (3 ml/kg b.w.) twice a week + methanolic extract of C. owariensis (300 mg/kg b.w.) daily for twenty eight (28) days. Phytochemical analysis of methanolic extract of C. owariensis was carried out using GC-MS. The body weight of study animals was measure at days 0, 7, 14, 21 and 28 of study. Then, kidney tissue of study animals was collected, weighed and processed for histopathological study. Tissue sections were stained using H & E, examined under microscope, photomicrographs were generated and observable histopathological changes were quantified using image-J software.

Phytochemical analysis of methanolic extract of C. owariensis showed abundance of phenolic compounds which may in turn confer antioxidant property on the extract. Results of this study also showed that treatment with extract helped to reduce body and tissue weight loss that follows exposure to CCl4. Also, treatment with the extract helped to reduce significantly (p < 0.05) renal histopathological changes following exposure to CCl4.

The methanolic extract of C. owariensis contains abundant phenolic compounds which confer antioxidant property that in turn mediate the nephroprotective potency of the extract against nephropathic effect of CCl4.

Keywords:
Cissampelos owariensis, carbon tetrachloride, nephroprotection, wistar rats.

Article Details

How to Cite
Omotoso, D. R., Lawal, O. S., Olatomide, O. D., & Okojie, I. G. (2020). Nephroprotective Effect of Cissampelos owariensis Extract on Renal Histomorphology of Wistar Rats during Exposure to Carbon Tetrachloride- induced Nephropathy. Asian Journal of Biology, 8(4), 1-10. https://doi.org/10.9734/ajob/2019/v8i430071
Section
Original Research Article

References

Yoshioka H, Usuda H, Fukuishi N, Nonogaki T, Onosaka S. Carbon tetrachloride-induced nephrotoxicity in mice is prevented by pretreatment with Zinc sulfate. Biol Pharmac Bull 2016; 39(6):1042–1046.

Safhi MM. Nephroprotective effect of Zingerone against CCl4-induced renal toxicity in Swiss Albino mice. Oxid Med Cell Longev; 2018.
DOI: 10.1155/2018/2474831

Khan MR, Siddique F. Antioxidant effects of Citharexylum spinosum in CCl4 induced nephrotoxicity in rat. Exp Toxicol Pathol 2012;64(4):349-355.
DOI: 10.1016/j.etp.2010.09.009

Khan MR, Zehra H. Amelioration of CCl4-induced nephrotoxicity by Oxalis corniculata in rat. Exp Toxicol Pathol. 2013;65(3):327-334.
DOI: 10.1016/j.etp.2011.11.007

Khan RA, Khan MR, Sahreen S, Bokhari J. Prevention of CCl4-induced nephrotoxicity with Sonchus asper in rat. Food Chem Toxicol. 2010;48(8-9):2469-246.
DOI: 10.1016/j.fct.2010.06.016

Okigbo RN, Eme UE, Ogbogu S. Biodiversity and conservation of medicinal and aromatic plants in Africa. Biotechnology and Molecular Biology Review. 2008;3(6):127–134.

Adelanwa EB, Tijjani AA. An ethno-medical survey of the flora of Kumbotso local government area of Kano State. Nigerian Journal of Pharmaceutical Sciences. 2013;12(1):1–9.

Akande R, Okwute SK, Iliya I, Efiom OO. Chemical constituents and anti-tuberculosis activity of the root extracts of Cissampelos owariensis (P. Beauv.) menispermaceae. African Journal of Pure and Applied Chemistry. 2013;7(1):21-30.

Krishnamoorthy K, Subramaniam P. Phytochemical profiling of leaf, stem and tuber parts of Solena amplexicaulis (Lam.) Gandhi using GC-MS. International Scholarly Research Notices; 2014.
DOI: 10.1155/2014/567409

Omotoso DR, Akinola AO, Daramola O, Uwagboe V, Onyewotu C. Assessment of gastroprotective activity of leaf extracts of Ageratum conyzoides Linn. using pyloric-ligation method in animal model. Int J Biomed Res. 2019;10(7):e5214.

Polterait O. Antioxidants and Free radical Scavengers of Natural Origin, Current Org. Chem. 1997;1:415-440.

Ayoola GA, Coker HAB, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC, Atangbayila TO. Phytochemical screening and anti-oxidant activities of some selected medicinal plants used for malaria therapy in South-Western Nigeria. Trop. J. Pharm. Res. 2008;7(3):1019-1024.

Olajide O, Orishadipe A, Fatokun O, Akande T, IIiya I. The chemical composition of essential oil from the root of Cissampelos owariensis (p.beauv) and free radical scavenging activities of its extracts. African Journal of Pure and Applied Chemistry. 2013;7(6):225–230.

Adjanohoun E, Ahyi MRA, Ake-Assi L, Elewude JA, Dramane K, Fadoju SO, Gbile ZO, Goudole E, Johnson CLA, Keita A, Morakinyo O, Ojewole JAO, Olatunji AO, Sofowora EA. Traditional medicine and pharmacopoeia. contribution to ethnobotanical floristic studies in Western Nigeria. Pub. Organization of African Unity, Scientific Technical and Research Commission Lagos, Nigeria. 1991;420.

Neuwinger HD. African traditional Medicine: A dictionary of plant use and applications. Medpharm Scientific Publishers, Stuttgart; 2000.

Schmelzer GH. Ressources végétales de l’Afrique tropicale. In: Plantes médicinales. PROTA, Wageningen, Netherlands. 2008;11(1).

Erhirhie OE, Moke EG, Chinwuba P. Cissampelos owariensis: Experimental review. The Pharma Innovation Journal. 2015;3(11):75–77.

Omotoso DR, Uwagbor V, Lawal OS, Olatomide OD, Okojie IG. Gross and histo-morphological study of anti-ulcerogenic effects of Cissampelos owariensis (P. Beauv.) Methanolic Extract in Wistar Rats. J Biomed Sci. 2019;8(3):15.

Khan RA, Khan MR, Sahreen S. CCl4-induced hepatotoxicity: protective effect of rutin on p53, CYP2E1 and the antioxidative status in rat. BMC Compl Alt Med. 2012; 12:178
DOI: 10.1186/1472-6882-12-178.

Ekeanyanwu RC, Udeme AA, Onuigbo AO, Etienajirhevwe OF. Anti-diabetic effect of ethanol leaf extract of Cissampelos owariensis (lungwort) on alloxan induced diabetic rats. African Journal of Biotechnology. 2012;11(25):6758–6762.

Omotoso DR, Okwuonu UC, Uwagbor V, Brown I, Oyaronbi OE. Immunohistochemical assessment of anti-proliferative potential of Cissampelos owariensis (P. Beauv.) methanolic extract in hepatic tissue of Wistar rats. Am J Res Med Sci. 2020;7(1):1-7.
DOI: 10.5455/ajrms.20190828104719

Craig GM, David JN. Natural product drug discovery in the next millennium. Pharm Biol. 2001;39(supplementary 1):8-17.

Omotoso DR, Akinola AO, Daramola O. Immunoexpression of cell proliferation (Ki-67) and tumor suppressor (p53) protein in hepatic tissue exposed to aqueous extract of Ageratum conyzoides Linn using rat model. Ann Med Biomed Sci. 2019;5(1): 8-12.

Abe R, Ohtani K. An ethnobotanical study of medicinal plants and traditional therapies on Batan Island, the Philippines. J Ethnopharmacol. 2013;145(2):554-565.

Abubakar A, Nazifi AB, Hassan FI, Duke KA, Edoh TD. Safety assessment of Chlorophytum alismifolium tuber extract (Liliaceae): Acute and sub-acute toxicity studies in Wistar rats. J Acute Dis. 2019; 8(1):21-27.

Aboonabi A, Rahmat A, Othman F. Effects of pomegranate on histopathology of liver and kidney on generated oxidative stress diabetic induced rats. J Cytol Histol. 2014; 6:1-5.

Ozturk F, Ucar M, Ozturk IC, Vardi N, Batcioglu K. Carbon tetrachloride-induced nephrotoxicity and protective effect of betaine in Sprague-Dawley rats. Urology. 2003;62(2):353-356.
DOI: 10.1016/s0090-4295(03)00255-3

Es.Haghi M, Dehghan G, Banihabib N, Zare S, Mikaili P, Panahi F. Protective effects of Conus mas fruit extract on carbon tetrachloride induced nephrotoxicity in rats. Ind J Nephrol. 2014;24(5):291-296.
DOI: 10.4103/0971-4065.133000

Awodele O, Adeneye AA, Aiyola SA, Benebo AS. Modulatory effect of Mangifera indica against carbon tetrachloride induced kidney damage in rats. Intersdiscip Toxicol. 2015;8(4):175-183.
DOI: 10.1515/intox-2015-0027

Adewole SO, Salako AA, Doherty OW, Naicker T. Effect of Melatonin on carbon tetrachloride-induced kidney injury in Wistar rats. Afr J Biomed Res. 2007;10: 153-164.

Sahareen S, Khan MR, Khan RA, Alkreathy HM. Protective effects of Carissa opaca fruits against CCl4-induced oxidative kidney lipid peroxidation and trauma in rat. Food Nutr Res. 2015;59:28438.
DOI: 10.3402/fnr.v59.28438

Dassarma B, Samanta S, Nandi DK, Gangopadhyay S, Protective effects of some Generally Recognized as Safe (GRAS) grade food preservatives against experimentally induced renal dysfunction. J Drug Deliv Therap. 2019;9(3-s):412-419.
DOI: 10.22270/jddt.v9i3-s.2871